Accelerate biotechnology

Upstream process development

We deliver scalable and robust GMP-compliant production processes for recombinant proteins.

Upstream process development for mammalian platforms

For small scale, high-throughput productions (microgram to milligram range of proteins), we typically apply our SCOUT® technology. This system integrates high-throughput cell culture using mini bioreactors (spin tubes; 2 – 20 mL working volume) with high throughput purification technology (spin-traps and spin-tubes) and matching analytical capabilities. Hereto, we have optimized CHO and HEK293 cell growth in our mini-bioreactors and have developed a number of media and medium feeds. To maximize the information generated during development, while limiting the number of experiments, a Design-of-Experiments (DOE) study format can be supported. For larger scale productions, we deploy a range of bioreactors up to 200 L depending on the development phase of the process. For suspension cultures, typically microwell plates, T-flasks, shake flasks or roller bottles in shake incubators, as well as WAVE Bioreactor™ systems and stirred single-use bioreactors are used, while for adherent cell cultures T-flasks, cell factories in static incubators or stirred tank reactors using microcarriers, iCELLis® systems or scale-X™ bioreactors in R&D and GMP. For these culture formats we can apply batch, fed-batch or perfusion culture protocols, depending project requirements.

All relevant in- and at line analytics are available to monitor and evaluate pivotal upstream process parameters to ensure optimal translation of the culture process to larger scales.

More information?

Downstream process development

Analytical development

Clinical manufacturing

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