SCOUT® technology for recombinant protein upstream development
Our SCOUT® technology has been used successfully to substantially speed up cell population doubling times of diverse cell lines. The SCOUT® technology has also been used to determine important protein production parameters. To deploy our SCOUT® technology, we have developed optimized protocols covering growth of diverse cell lines in the SCOUT® bioreactors, including CHO and HEK293 cell lines. As such, we have generic upstream processes for different cell lines, which we use as a foundation to start with the development of a product specific process. Hereto, a panel of commercially available production media and generic feed strategies are available for testing in mini-bioreactors to optimize cell growth, cell population doubling times, and to increase cell densities. One of the great advantages of our SCOUT® technology, apart from speed, cost-reduction, and versatility, is our experience that the optimal upstream parameters identified from our SCOUT® experiments can be seamlessly extrapolated to scale up to larger manufacturing bioreactors.
SCOUT® technology for recombinant protein downstream development
The cell culture capabilities that are part of the SCOUT® technology are matched with high-throughput purification technology. Purification is performed using commercially available 96-well plates, spin-traps and spin-filters and which are packed in-house with various resins to generate custom-made, product-specific, high-throughput purification tools when necessary. High-throughput screening of novel resins to identify binding and elution (using 96-well plate or spin-traps) is part of our repertoire to quickly develop reliable protocols for pharmaceutical grade purification. Naturally, the SCOUT® technology is backed by a wide range of assays ensuring in-process control, anchoring SCOUT® technology as a complete laboratory tool for the rapid and robust development of manufacturing protocols for recombinant proteins.